Methods Used For Celiac Disease Diagnostic Test


celiac disease diagnostic test

Celiac Disease Diagnostic Test is an important part of the disease. This disease is a genetically defined hypersensitivity to gluten, a protein found in wheat, rye and barley. In celiac sprue, a similar condition occurs which is referred to as sprue alopecia. This disease is characterized by abnormal growth of intestinal villi and chronic diarrhea that is caused by adherence of these villi to the intestinal wall. The major symptom of this condition is diarrhea.

Celiac Disease Diagnostic Test Involves The Use Of Transgenic Mouse Models

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Celiac Disease Diagnostic Testing involves the use of transgenic mouse models in order to show that gluten can be absorbed by cells and that it affects cell-potential. In order to show that gluten can be absorbed by the small intestine, intracellular fluorescent microscope (CTF) or optical fluorescent microscope (OFLM) is used. For a conclusive diagnosis, biopsy of the small intestine from patients with celiac disease is required.

Another important component of the celiac disease diagnostic test is a test for gluten-specific t-cells. This is also known as very negative. This is a special type of t-cell that is specific to gliadin. This enables the doctor to identify whether a patient has celiac disease by the absence or presence of the specific motif, called the t-cells receptor (TCR). It is composed of residues that are complementary to glutenin and gliadin.

In addition to the t-cells, an immune response is evaluated. This is done through a procedure known as a challenge-response. This procedure is done after exposing the small intestines to gluten and gauging the mucus production and the immune response. Blood samples are taken and tested for the levels of the humic acid marker hla-humic acid.

PCR And Gene Array Expression Profiling Technologies

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Lastly, the genes for the gluten-specific cells isolated using fecal smears are evaluated using PCR and Gene Array Expression Profiling technologies. Usually, one set of genes is mixed with a control sample and then amplified in order to obtain the desired level of expression. The different sets of genes are used depending on whether the individual has celiac disease or not. Usually, three sets of genes are evaluated. One for the regulatory genes, one for the innate immune genes and one for the vascular genes.

Next, PCR amplifies the fragments of the four pairs of transmembrane proteins. These fragments are then inserted into the gene-containing sequence of the T-cells. The t-cells then generate mature epitopes. This process allows researchers to study the molecular basis of the T-cells’ functionality. This is because T-cells recognize common allergens such as wheat and barley.

Based on the results obtained from this experiment, a gluten-specific cell locus (gluten-a gene pair) is then found. Based on the results obtained from this experiment, a new method called DNA microarray hybridization is used. This method relies on PCR amplification of different fragments of the four pairs of transmembrane proteins. These fragments are then put together using DNA templates generated from the genes for the T-cells and other molecules. The resulting ensemble is called a complex. Based on the results obtained from the hybridization analysis, the presence of a new locus for celiac disease is then established.

Novel HLA-DR4

Another important result obtained from this study is that a novel HLA-DR4 domain is found which is composed of tandem repeats. This domain binds to different IgE molecules. It then initiates a t-cell response. In addition, a unique T-cell receptor binding profile has also been discovered. Based on these results, it was concluded that the HLA-DR4 domain and the IgE molecules play an important role in celiac disease.

Celiac disease diagnostic testing using genetic data analysis was carried out using intestinal biopsies from patients with celiac sprue. An important finding from these biopsies is that small intestine mucosa appears to be uninfected. In fact, there is no evidence of villi inflammation. Hence, these biopsies can serve as diagnostic markers for the potential presence of celiac sprue in the small intestine.

A Celiac Disease Diagnostic Test study also indicates that the amino acid amylase (Aminase) plays a key role in gluten digestion and in breaking down of glucose. Moreover, amino acid lysine, together with cysteine, appears to be the best candidates to regulate insulin release. This suggests that treatment with an anti-gluten diet is necessary to prevent the occurrence of insulin resistance and diabetes. A gluten-free diet is indicated for the management of diabetes.

Summing Up

Celiac Disease Diagnostic Testing has now advanced to the point that analysis of plasma and blood samples can provide results in less than 30 minutes. Amino acid profiles are highly consistent between subjects with gluten-intolerance and non-gliadin-intolerant subjects. In order to facilitate the use of amino acid profiles for analyzing the genetic background of patients and for the prediction of the effects of treatments on this disease, several parameters have been established which allow the calculation of the percentage of protein and fat tissue in the various tissues of the body. These values are highly correlated between patients with gluten-intolerance and non-gliadin-intolerant subjects.

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